|
|
Characterization and application of microwave plasma on wound healing
Smejkalová, Kateřina ; Skoumalová, Petra (referee) ; Krčma, František (advisor)
The aim of the Master thesis is the investigation of the influence of microwave discharge for skin wound healing. Microwave discharge used for this work was argon microwave plasma generated by the surface wave and direct vortex torch. The theoretical part is focused on basic information about plasma and processes that occur in plasma discharge under specific conditions. Plasma generates various active particles such as hydroxyl radicals, nitric oxide radicals, excited nitrogen molecules, atomic nitrogen, argon and oxygen. All of these particles together with plasma generated photons are usable in biomedical applications and summary of them is shown in the theoretical part. The experimental part is focused on the comparison of torch discharge and microwave plasma generated surface wave in skin wound healing. The model wounds on laboratory mousses were treated by plasma and wound healing was examined during 3 weeks after the plasma treatment. Both plasma systems showed healing acceleration. Application of torch discharge was proved to be the most effective method in the healing of skin defects. Additionally, determination of active particles was taken by optical emission spectroscopy. Based on these measurements, plasma parameters were determined: electron temparutare, rotational and vibrational temperatures. To determine role of different plasma active species, the treatment of indigo coloured artificial skin model was treated under various conditions by both plasma systems. Results show that the direct interaction between plasma particles is the main effect, role of radiation, only, is more or less negligible. Finally, the plasma vortex system was visualized using fast camera at selected powers and gas flows.
|
|
|
Characterization of microwave plasma jet generated in argon-oxygen mixtures
Smejkalová, Kateřina ; Mazánková, Věra (referee) ; Krčma, František (advisor)
The optical diagnostics of microwave plasma torch operating at the frequency of 2,45 GHz is a subject of this bachelor thesis. Microwave plasma is generated using the surfatron resonator in argon with oxygen admixture (up to 1,7 %) at the constant argon flow of 3 Slm. The theoretical part gives the basic information about plasma and processes running under plasma conditions. Various active particles such as hydroxyl radicals, nitric oxides, excited nitrogen molecules, nitrogen, argon and oxygen atoms are generated in the discharge. All these particles together with the plasma generated photons can be used in biomedical applications that are briefly reviewed in the theoretical part, too. The visual observation of oxygen admixture on the discharge and the optical emission spectroscopy based determination of the active particles presence along the discharge and post-discharge axis is the subject of experimental part. Based on it, the selected plasma parameters like electron, rotational and vibrational temperatures were calculated.
|
|
|
Optimization of distribution of active particles generated by low temperature plasma on biopolymer surfaces
Krupičková, Lucie ; Kozáková, Zdenka (referee) ; Krčma, František (advisor)
This thesis is focused on the optimization of the distribution of active particles (RONS), generated by the low temperature plasma, on the biopolymer surfaces. The theoretical part characterizes plasma and its active particles. Furthermore, this chapter summarizes its applications in medicine and food industry, also a review of different skin models is listed here. The last section is focused on the skin anatomy and the characterization of selected microorganisms C. glabrata, E. coli and S. epidermidis. In the experimental part, selected biopolymers with specific dyes for detection of active particles were prepared. Furthermore biopolymers were spot treated under different conditions leading to color changes in all biopolymers. This test confirmed a presence of active particles in the plasma. After that, the optimal parameters for active particles distribution over the entire surface were found. Also an experiment, which allowed the UV light to pass through but prohibited the passage of active particles, was made. In this experiment, no color change was noticed, which means, that the reaction of active particles with the colored biopolymer is responsible for the color change. The optimized parameters were used for treatment of agar plates with monoculture of C. glabrata, E. coli, S. epidermidis and with mixed culture C. glabrata + E. coli. Two different plasma torches were used – unipolar microwave discharge torch and surface wave microwave discharge jet. Treated samples were photographed after incubation. Software Aurora then calculated the surface area which was covered by the microbial culture. Based on the data, the microbial reducion was evaluated in comparison with untreated samples. The unipolar microwave discharge torch achieved higher efficiency than the surface wave microwave discharge jet, for all tested microorganisms.
|
|
|
Characterization and application of microwave plasma on wound healing
Smejkalová, Kateřina ; Skoumalová, Petra (referee) ; Krčma, František (advisor)
The aim of the Master thesis is the investigation of the influence of microwave discharge for skin wound healing. Microwave discharge used for this work was argon microwave plasma generated by the surface wave and direct vortex torch. The theoretical part is focused on basic information about plasma and processes that occur in plasma discharge under specific conditions. Plasma generates various active particles such as hydroxyl radicals, nitric oxide radicals, excited nitrogen molecules, atomic nitrogen, argon and oxygen. All of these particles together with plasma generated photons are usable in biomedical applications and summary of them is shown in the theoretical part. The experimental part is focused on the comparison of torch discharge and microwave plasma generated surface wave in skin wound healing. The model wounds on laboratory mousses were treated by plasma and wound healing was examined during 3 weeks after the plasma treatment. Both plasma systems showed healing acceleration. Application of torch discharge was proved to be the most effective method in the healing of skin defects. Additionally, determination of active particles was taken by optical emission spectroscopy. Based on these measurements, plasma parameters were determined: electron temparutare, rotational and vibrational temperatures. To determine role of different plasma active species, the treatment of indigo coloured artificial skin model was treated under various conditions by both plasma systems. Results show that the direct interaction between plasma particles is the main effect, role of radiation, only, is more or less negligible. Finally, the plasma vortex system was visualized using fast camera at selected powers and gas flows.
|
|
|
Characterization of microwave plasma jet generated in argon-oxygen mixtures
Smejkalová, Kateřina ; Mazánková, Věra (referee) ; Krčma, František (advisor)
The optical diagnostics of microwave plasma torch operating at the frequency of 2,45 GHz is a subject of this bachelor thesis. Microwave plasma is generated using the surfatron resonator in argon with oxygen admixture (up to 1,7 %) at the constant argon flow of 3 Slm. The theoretical part gives the basic information about plasma and processes running under plasma conditions. Various active particles such as hydroxyl radicals, nitric oxides, excited nitrogen molecules, nitrogen, argon and oxygen atoms are generated in the discharge. All these particles together with the plasma generated photons can be used in biomedical applications that are briefly reviewed in the theoretical part, too. The visual observation of oxygen admixture on the discharge and the optical emission spectroscopy based determination of the active particles presence along the discharge and post-discharge axis is the subject of experimental part. Based on it, the selected plasma parameters like electron, rotational and vibrational temperatures were calculated.
|
guest ::
